Effectiveness of Custard Apple (Annona squamosa) Seed Extract in Treating Pediculosis Capitis


  • Jellemer M. Benitez Cebu Normal University
  • Chelsea V. Cimafranca Cebu Normal University
  • Louisse Phillina Paz V. Gimenez Cebu Normal University
  • Jezyl C. Cutamora Cebu Normal University




Pediculosis capitis, Annona squamosa, permethrin, pediculicide, extract


Pediculosis capitis infestation, commonly known as head lice, a worldwide public health concern affecting individuals of all age groups, and prevalence in the general population can be as high as 40 percent. The main task attained in the study was the determination of the pediculicidal effects of Annona squamosa seed extract of different concentrations. Methodology utilized a true experimental design done in 3 trials; 10 live head lice were observed under each concentration of 25%, 50%, 75% atis seeds extract for the experimentation and 75% permethrin concentration as controlled group. In vitro testing was used and the subjects were placed in prepared petri dishes for experimentation. Data results found out that there is p-value of 0.067 at α = 0.05 for mean time elapsed (secs) for 100% head lice mortality. Significant p-level of 0.002 for pediculicidal activity for the first 5 minutes has been noted. The researchers derived up with a conclusion that Custard Apple (Annona squamosa) seed extract in 75%, 50% and 25% concentration is as effective as Commercial Pediculicide (Permethrin) in killing pediculosis Capitis. 50% concentration has comparable results to Permethrin and is the most effective due to its earlier onset of pediculicidal activity.

Author Biography

Jezyl C. Cutamora, Cebu Normal University

is a faculty member of Cebu Normal University – College of Nursing both in the undergraduate and graduate studies since 2005.She obtained a degree in Master in Nursing
major in Medical-Surgical Nursing in 2008 at Cebu Normal University. She is also the College Research Coordinator since 2009 and is currently pursuing her Ph.D in education major in Research and Evaluation in the same institution. She has authored and co-authored published researches in international and national category. She received research awards for publication and presentation of research articles.


AlBashtawy, M. & Hasna, F. (2012). Pediculosis capitis among primary-school children in Mafraq Governorate, Jordan. Eastern Mediterranean Health Journal, 18(1), 43-48.  

Dodd, C. S. (2006). Interventions for treating head lice (Review). Cochrane Database of Systematic Reviews, (4), CD001165. Retrieved from http://onlinelibrary.wiley.com/doi/10.1002/14651858.CD001165.pub2/epdf

Intaranongpai, J., Gritsanapan, W. & Chavasiri, W. (2006). Anti-head lice effect of Annona squamosa seeds. Southeast Asian Journal of Tropical Medicine Public Health, 37(3), 532- 535. Retrieved from http://www.tm.mahidol.ac.th/seameo/2006_37_3/16-3806.pdf

Kosalge, S. B. & Fursule, R. A. (2009). Investigation of licicidal activity of some plants from Satpuda Hills. International Journal of PharmTech Research, 1(3), 564-567. Retrieved from http://sphinxsai.com/ptvol3/pt=30,%20satish%20kosalge%20(564-567).pdf

Meinking, T. L, Entzel, P., Villar, M. E., Vicaria, M., Lemard, G. A. & Porcelain, S. L. (2000). Comparative efficacy of treatments for Pediculosis capitis infestations: Update 2000. Arch Dermatol, 137(3), 287-292. doi:10.1001/archderm.1986.01660150045013

Parvin, S., Islam, E., Rahman, M. & Haque, E. (2003). Toxicological evaluation of annotemoyin-1 isolated from Annona squamosa Linn.on on Long Evans rats. Pakistan Journal of Biological Science, 6(18), 1593-1596. Retrieved from http://docsdrive.com/pdfs/ansinet/pjbs/2003/1593-1596.pdf

Public Health Medicine Environmental Group. (2008). Head lice: Evidence-based guidelines based on the the Stafford Report 2008 Update. MeReC Bulletin, 18(5).

Tiangda, C. H., Gritsanapan, W., Sookvanichsilp, N. & Limchalearn, A. (2000). Anti-headlice activity of a preparation of Annona squamosa seed extract. Southeast Asian Journal of Tropical Medicine and Public Health, 31, 174-177.

Toynton, K., Luukinen, B., Buhl, K. & Stone, D. (2009). Permethirn technical fact sheet. Corvallis, OR: National Pesticide Information Center. Retrieved last March 23, 2013, from http://npic.orst.edu/factsheets/Permtech.pdf

Vander Stichele, R.H., Dezeure, E.M., & Bogaert, M.G. (1995). Systematic review of clinical efficacy of topical treatments for head lice. BJM, 311(7005), 604-8. doi: https://doi.org/10.1136/bmj.311.7005.604

Yagumyum, P. (2005). Efficacy of atis (Annona squamosa) as a pediculicide: An in vitro study. Philippine Scientific Journal, 38(1), 23-30.

Yapchiongco, R. (2010, April 5). Attack of the lice [Web log post]. Retrieved last February 16, 2013, from http://www.thepoc.net/thepoc-features/health-and-wellness/health-and-fitness-features/5648-how-to-deal-with-head-lice-nits-treatment-prevention.html




How to Cite

Benitez, J. M., Cimafranca, C. V., Gimenez, L. P. P. V., & Cutamora, J. C. (2015). Effectiveness of Custard Apple (Annona squamosa) Seed Extract in Treating Pediculosis Capitis. Recoletos Multidisciplinary Research Journal, 3(2). https://doi.org/10.32871/rmrj1503.02.08