The Antiangiogenic Activity of the Pereskia grandifolia (Seven Star Needle) Crude Leaf Extract Using the Chick Embryo Chorioallantoic Membrane (CAM) Assay
DOI:
https://doi.org/10.32871/rmrj25si.t2414Keywords:
Pereskia grandifolia, antiangiogenic activity, chorioallantoic membrane (CAM) assay, drug therapy, dexamethasone, flavonoidsAbstract
Background: The study focused on the anticancer potential of Pereskia grandifolia, specifically its antiangiogenic properties.
Methods: Soxhlet extraction and rotary evaporation yielded a crude leaf extract containing flavonoids, phenols, tannins, steroids, and alkaloids. An ex-ovo Chorioallantoic Membrane (CAM) Assay was performed on twenty-one 4-day-old chicken embryos, divided into three groups: the experimental group (40.5 mg/50 mcL crude extract), positive control (7.5 mcg/50 mcL dexamethasone), and negative control (50 mcL distilled water).
Results: The experimental group exhibited the highest inhibition of blood vessel length (43.99%), significantly greater than the positive control group (28.51%) and negative control group (0%). Statistical analysis confirmed significant differences in blood vessel length inhibition (t = 2.590, p = 0.024), number (F = 93.415, p < 0.001, w² = 0.80), and vessel density (F = 94.343, p < 0.001).
Conclusion: These findings suggest Pereskia grandifolia's potential as an antiangiogenic agent for cancer therapy.
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