Bio-efficacy of Trichoderma harzianum Rifai and Botanical Extracts against Major Fungal Pathogens of Tamarind (Tamarindus indica L.) under Field Condition

Authors

DOI:

https://doi.org/10.32871/rmrj2311.01.02

Keywords:

biocontrol, botanical extracts, major fungal diseases, tamarind, Philippines

Abstract

Domestic production of tamarind has decreased in the country due to diseases. Tests were made on the individual efficacy of botanical extracts like plant growth promoter (PGP) and oriental herb nutrient (OHN), as well as the Trichoderma harzianum Rifai as a possible biocontrol agent against major fungal tamarind diseases. Using Randomized Complete Block Design (RCBD), a field setup was established with five treatments: untreated (negative control), synthetic fungicide, OHN, PGP, and T. harzianum having three replications per treatment. Results revealed that T. harzianum is an effective biocontrol agent against major fungal tamarind diseases. PGP having similar efficiency with T. harzianum significantly inhibited the infection and provided immediate defense from diseases. OHN is less effective due to delayed reduced infection. The research output is a significant help in the management of various diseases that is cost-effective and safe.

Author Biographies

Mary Grace Gatan, Pampanga State Agricultural University, Magalang, Pampanga, Philippines

Faculty
College of Agriculture Systems and Technology, Pampanga State Agricultural University, Magalang, Pampanga, Philippines

Director
Research and Development Department, Pampanga State Agricultural University, Magalang, Pampanga, Philippines

Menrado Gatan, Pampanga State Agricultural University, Magalang, Pampanga, Philippines

College of Agriculture Systems and Technology, Pampanga State Agricultural University, Magalang, Pampanga, Philippines

Research and Development, Department, Pampanga State Agricultural University, Magalang, Pampanga, Philippines

Cesar Orpiada, Pampanga State Agricultural University, Magalang, Pampanga, Philippines

Research and Development Department, Pampanga State Agricultural University, Magalang, Pampanga, Philippines

References

Abdulkadir, A., Hayatu, M., Sani, L. A., & Ahmed, H. (2022). Biocontrol ability of Trichoderma harzianum on growth, disease incidence and yield of selected cowpea varieties (Vigna unguiculata L.) infested with Colletotrichum lindemuthianum. Bima Journal of Science and Technology, 6(2), 65-76. https://doi.org/10.56892/bimajst.v6i02.356

Abdullahi, A., Khairulmazmi, A., Yasmeen, S., Ismail, I. S., Norhayu, A., Sulaiman, M. R., Ahmed, O. H., & Ismail, M. R. (2020). Phytochemical profiling and antimicrobial activity of ginger (Zingiber officinale) essential oils against important phytopathogens. Arabian Journal of Chemistry, 13(11), 8012-8025. https://doi.org/10.1016/j.arabjc.2020.09.031

Agi, V., & Azike, C. (2019). Antifungal action of garlic (Allium sativum) and ginger (Zingiber officinale) on some pathogenic fungi. Asian Journal of Research Biochemistry, 4(4), 1-6. https://doi.org/10.9734/ajrb/2019/v4i430075

Agrios, G. N. (1988). Plant pathology (3rd ed.). Academic Press.

Anal, A. K. D., Rai, S., Singh, M., & Solanki, M. K. (2020). Plant mycobiome: Current research and applications. In M. Solanki, P. Kashyap, & B. Kumari (Eds.), Phytobiomes: Current insights and future vistas (81-104). Springer. https://doi.org/10.1007/978-981-15-3151-4_4

Castleman, M. (2010). The new healing herbs: The essential guide to more than 125 of nature’s most potent herbal remedies. Rodale Press.

Chang, K. C. S., McGinn, J. M., Weinert Jr., E., Miller, S., Ikeda, D. M., & DuPonte, M. W. (2014). Natural farming: Oriental herbal nutrient. College of Tropical Agriculture and Human Resources (CTAHR). https://www.ctahr.hawaii.edu/oc/freepubs/pdf/sa-11.pdf

Cho, J. Y., Choi, G. J., Lee, S. W., Jang, K. S., Lim, H. K., Lim, C. H., Cho, K.-Y., & Kim, J.-C. (2006). Antifungal activity against Colletotrichum spp. of curcuminoids isolated from Curcuma longa L. rhizomes. Journal of Microbiology and Biotechnology, 16(2), 280-285. https://www.researchgate.net/publication/283821700_Antifungal_activity_against_Colletotrichum_spp_of_curcuminoids_isolated_from_Curcuma_longa_L_rhizomes

Chow, J. (2002). Probiotics and prebiotics: A brief overview. Journal of Renal Nutrition, 12(2), 76–86. https://doi.org/10.1053/jren.2002.31759

Cooke, R. C., & Whipps, J. M. (1993). Ecophysiology of fungi. Blackwell Scientific.

Darmadi, A. A. K., Suriani, N. L., Darmayasa, I. B. G., Bagus, I., Suaskara, M., Gari, N. M., & Fudholi, A. (2021). Cinnamon leaf extract to control anthracnose disease on Chilli plants in Bali: A novel and new potential. International Journal of Pharmaceutical Research, 13(1), 1006-1015. https://simdos.unud.ac.id/uploads/file_penelitian_1_dir/ecb1a9d3943a8fb2e24aa53248f3f927.pdf

Darmadi, A. A. K., Suriani, N. L., Sudirga, S. K., & Khalimi, K. (2019). First study on Fusarium equiseti: Causes fusarium wilt in tomato crop in Bali, Indonesia. SABRAO Journal of Breeding & Genetics, 51(4), 442-450. https://sabraojournal.org/wp-content/uploads/2020/01/SABRAO-J-Breed-Genet-514-442-450-Darmadi.pdf

Davari, M., & Ezazi, R. (2022). Mycelial inhibitory effects of antagonistic fungi, plant essential oils and propolis against five phytopathogenic Fusarium species. Archives of Microbiology, 204(8), 480. https://doi.org/10.1007/s00203-022-03102-6

El-Siddig, K., Gunasena, H. P. M., Prasad, B. A., Pushpakumara, D. K. N. G., Ramana, K. V. R., Vijayanand, P., & Williams, J. T. (2006). Tamarind: Tamarindus indica L. Southampton Centre for Underutilised Crops. https://books.google.com.ph/books?id=QhtZLMVPLIIC&lpg=PR1&dq=Tamarind%3A%20Tamarindus%20Indica%20L%20(Vol.%201).%20Crops%20for%20the%20Future.%20International%20Centre%20for%20Underutilised%20Crops%20University%20of%20Southampton%2C%201%3A1&pg=PR1#v=onepage&q&f=false

Elnahal, A. S. M., El-Saadony, M. T., Saad, A. M., Desoky, E-S. M., El-Tahan, A. M., Rady, M. M., AbuQamar, S. F., & El-Tarabily, K. A. (2022). The use of microbial inoculants for biological control, plant growth promotion, and sustainable agriculture: A review. European Journal of Plant Pathology, 162, 759–792. https://doi.org/10.1007/s10658-021-02393-7

Gatan, M. G. (2021). Identification and assessment of major phytopathogenic fungi of tamarind leaves in Central Luzon, Philippines. http://dx.doi.org/10.2139/ssrn.3937830

Gawarkar, S. V., Deshmukh, R. G., Matte, A. D., & Patil, M. J. (2022). In vitro management of Aspergillus niger of Sesamum (Sesamum indicum). The Pharma Innovation Journal, 11(12), 768-770. https://www.thepharmajournal.com/archives/2022/vol11issue12/PartJ/11-11-380-789.pdf

Gwa, V. I., & Ekefan, E. J. (2017). Fungal organisms isolated from rotted white yam (Dioscorea rotundata) tubers and antagonistic potential of Trichoderma harzianum against Colletotrichum species. Agricultural Research & Technology Open Access Journal, 10(3), 58-67. https://juniperpublishers.com/artoaj/pdf/ARTOAJ.MS.ID.555787.pdf

Habsah, M., Amran, M., Mackeen, M. M., Lajis, N. H., Kikuzaki, H., Nakatani, N., Rahman, A. A., Ghafar, & Ali, A. M. (2000). Screening of Zingiberaceae extracts for antimicrobial and antioxidant activities. Journal of Ethnopharmacology, 72(3), 403-410. https://doi.org/10.1016/S0378-8741(00)00223-3

Hafez, Y. M., El-Nagar, A. S., Elzaawely, A. A., Kamel, S., & Maswada, H. F. (2018). Biological control of Podosphaera xanthii the causal agent of squash powdery mildew disease by upregulation of defense-related enzymes. Egyptian Journal of Biological Pest Control, 28(1), 57. https://doi.org/10.1186/s41938-018-0058-8

Harrison, J. G., Lowe, R., & Williams, N. A. (1994). Humidity and fungal diseases of plants–problems. In J. P. Blakeman & B. Williamson (Eds.), Ecology of plant pathogens (79-97). CAB International.

Hojo, H., & Sato, J. (2002). Antifungal activity of Licorice (Glycyrrhiza glabra) and potential applications in beverage. Foods & Food Ingredients Journal of Japan, (203), 27-33.

Hussain, I., Alam, S. S., Khan, I., Shah, B., Naeem, A., Khan, N., Ullah, W., Iqbal, B., Adnan, M., Junaid, K., Shah, S. R. A., Ahmed, N., & Iqbal, M. (2016). Studies on biological management of fusarium wilt of tomato. Journal of Entomology and Zoology Studies, 4(2), 525-528. https://www.entomoljournal.com/archives/2016/vol4issue2/PartH/4-3-59.pdf

Jayaprakasha, G. K., & Rao, L. J. (2011). Chemistry, biogenesis, and biological activities of Cinnamomum zeylanicum. Critical Reviews in Food Science and Nutrition, 51(6), 547–562. https://doi.org/10.1080/10408391003699550

Maekawa, L. E., Valera, M. C., de Oliveira, L.D., Carvalho, C. A. T., Camargo, C. H. R., & Jorge, A. O. C. (2013). Effect of Zingiber officinale and propolis on microorganisms and endotoxins in root canals. Journal of Applied Oral Science, 21(1)25–31. http://dx.doi.org/10.1590/1678-7757201302129

Ming, L. J., & Yin, A. C. (2013). Therapeutic effects of glycyrrhizic acid. Natural Product Communications, 8(3), 415–418. https://doi.org/10.1177/1934578X1300800335

Moenne, A., & González, A. (2021). Chitosan-, alginate-carrageenan-derived oligosaccharides stimulate defense against biotic and abiotic stresses, and growth in plants: A historical perspective. Carbohydrate Research, 503, 108298. https://doi.org/10.1016/j.carres.2021.108298

Muliani, Y., Irmawatie, L., Sukma, S. M., Srimurni, R. R., Adviany, I., Ustari, D., & Milani, M. N. (2022). Antagonism Trichoderma harzianum rifai in suppresing the intensity of Antraknosa (Colletotrichum capcisi Sydow.) disease. Al-Hayat: Journal of Biology and Applied Biology, 5(1), 75-88. https://doi.org/10.21580/ah.v5i1.13546

Panwar, V., Aggarwal, A., Singh, G., Verma, A., Sharma, I., & Saharan, M. S. (2014). Efficacy of foliar spray of Trichoderma isolates against Fusarium graminearum causing head blight of wheat. Journal of Wheat Research, 6(1), 59-63. https://sawbar.in/wp-content/uploads/2018/07/41949-97915-1-SM.pdf

Petnual P., Sangvanich, P., & Karnchanatat, A. (2010). A lectin from the rhizomes of turmeric (Curcuma longa L.) and its antifungal, antibacterial, and α-glucosidase inhibitory activities. Food Science and Biotechnology, 19, 907-916. https://doi.org/10.1007/s10068-010-0128-5

Philippine Statistics Authority. (n.d.). 2016-2020 crops statistics of the Philippines. https://psa.gov.ph/sites/default/files/Crops%20Statistics%20of%20the%20Philippines%202016-2020.pdf

Redo, M. C., Rios, J. L., & Villar, A. (1989). A review of some antimicrobial compounds isolated from medicinal plants reported in the literature 1978-1988. Phytotherapy Research, 3(4), 117-125. https://doi.org/10.1002/ptr.2650030402

Ren, X., Branà, M. T., Haidukowski, M., Gallo, A., Zhang, Q., Logrieco, A. F., Li, P., Zhao, S., & Altomare, C. (2022). Potential of Trichoderma spp. for biocontrol of aflatoxin-producing Aspergillus flavus. Toxins, 14(2), 86. https://doi.org/10.3390/toxins14020086

Sani, M. N. H., & Yong, J. W. H. (2022). Harnessing synergistic biostimulatory processes: A plausible approach for enhanced crop growth and resilience in organic farming. Biology, 11(1), 41. https://doi.org/10.3390/biology11010041

Saran, M. K., Ram, D., Verma, J. R., & Choudhary, A. (2021). Effect of different plant extracts and bio-agents against collar rot of groundnut caused by Aspergillus niger van tiegham. Biopesticides International, 17(2). 159-162. https://connectjournals.com/02196.2021.17.159

Sarfraz, M., Nasim, M. J., Jacob, C., & Gruhlke, M. C. H. (2020). Efficacy of allicin against plant pathogenic fungi and unveiling the underlying mode of action employing yeast based chemogenetic profiling approach. Applied Sciences, 10(7), 2563. https://doi.org/10.3390/app10072563

Sarker, S. D., & Nahar, L. (2004). Natural medicine: The genus Angelica. Current Medicinal Chemistry, 11(11), 1479–1500. https://doi.org/10.2174/0929867043365189

Savatin, D. V., Gramegna, G., Modesti, V., & Cervone, F. (2014). Wounding in the plant tissue: The defense of a dangerous passage. Frontiers in Plant Science, 5, 470. https://doi.org/10.3389/fpls.2014.00470

Shoresh, M., & Harman, G. E. (2008). The molecular basis of shoot responses of maize seedlings to Trichoderma harzianum T22 inoculation of the root: A proteomic approach. Plant Physiology, 147(4), 2147-2163. https://doi.org/10.1104/pp.108.123810

Sivan, A., & Chet, I. (1986). Biological control of Fusarium spp. in cotton, wheat and muskmelon by Trichoderma harzianum. Journal of Phytopathology, 116(1), 39-47. https://doi.org/10.1111/j.1439-0434.1986.tb00892.x

Soares, F., Fernandes, C., Silva, P., Pereira, L., & Gonçalves, T. (2016). Antifungal activity of carrageenan extracts from the red alga Chondracanthus teedei var. lusitanicus. Journal of Applied Phycology, 28(5), 2991-2998. https://doi.org/10.1007/s10811-016-0849-9

Talley, S. M., Coley, P. D., & Kursar, T. A. (2002). The effects of weather on fungal abundance and richness among 25 communities in the Intermountain West. BMC Ecology, 2(7), 1-11. https://doi.org/10.1186/1472-6785-2-7

Teles, A., dos Santos, B. A., Ferreira, C. G., Mouchreck, A. N., Calabrese, K. S., Abreu-Silva, A. L., & Almeida-Souza, F. (2020). Ginger (Zingiber officinale) antimicrobial potential: A review. In H. Wang (Ed.), Ginger cultivation and its antimicrobial and pharmacological potentials. IntechOpen. https://doi.org/10.5772/intechopen.89780

Triasih, U., Abadi, A. L., Muhibbudin, A., & Widyaningsih, S. (2022, October 19). Uji beberapa jamur antagonis terhadap Colletotrichum gloeosporiodes penyebab penyakit busuk buah apel manalagi (Malus sylvestris) secara in vitro [Conference session]. Agropross: National Conference Proceedings of Agriculture, Indonesia. https://doi.org/10.25047/agropross.2022.309

Yadav, S. K., Sah, A. K., Jha, R. K., Sah, P., & Shah, D. K. (2013). Turmeric (curcumin) remedies gastroprotective action. Pharmacognosy Reviews, 7(13), 42–46. https://doi.org/10.4103/0973-7847.112843

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Published

2023-04-03

How to Cite

Gatan, M. G., Bulanadi, A., Limpin, M. A. R., Gatan, M., & Orpiada, C. (2023). Bio-efficacy of Trichoderma harzianum Rifai and Botanical Extracts against Major Fungal Pathogens of Tamarind (Tamarindus indica L.) under Field Condition. Recoletos Multidisciplinary Research Journal, 11(1), 41–53. https://doi.org/10.32871/rmrj2311.01.02

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